Fig. 1: Copper chelation potentiates antitumor activity of anti-GD2 immunotherapy.

a Experimental design and dosing strategy. Schematic created in BioRender. Vittorio, O. (2024). BioRender.com/c53h577. b Individual tumor kinetics of Th-MYCN mice. c Kaplan–Meier survival curves of Th-MYCN mice presented in (b). Statistical pairwise comparisons were calculated using a two-tailed Mantel–Cox log-rank test with p values displayed in the figure. For (b) and (c), data are n = 11 (Saline + IgG2a) or n = 10 (all other groups) biological replicates, one independent experiment. d Representative images of merged OPAL multiplex immunofluorescence spectra depicting the tumoral distribution of NCR1⁺ natural killer cells (red), CD8⁺ cytotoxic T cells (yellow), CD11b⁺ myeloid (white) and DAPI nuclei stain (blue) in Th-MYCN neuroblastoma tumor tissue 14 days post-treatment. Scale bar, 100 µm. e Immune cell quantification of (d) as positive counts per 1000 nuclei. Significance was calculated using a two-tailed Mann–Whitney U test with p values displayed in figure. For (e), Data are mean ± SEM n = 4 (Saline + IgG2a) or n = 3 (all other groups) biological replicates with a minimum of two technical replicates, one independent experiment. Abbreviations: IP intraperitoneal, p.o. orally. Source data are provided as a Source Data file.