Fig. 3: Copper chelation reinvigorates antitumor immunity via pro-inflammatory signaling.
From: Copper chelation redirects neutrophil function to enhance anti-GD2 antibody therapy in neuroblastoma
a Representative images of a tissue microarray containing control and TEPA-treated Th-MYCN tumor cores (n = 10/treatment, biological replicates in technical duplicate, total n = 20 cores; one independent experiment) and resected after 0, 3 or 7 days for NanoString GeoMx Digital Spatial Profiling, stained with fluorescently conjugated antibodies to PanCK (red), smooth muscle actin (yellow), CD45 (green) with DAPI nuclei stain (blue). Scale bar, 300 µm. One independent experiment. b Sankey diagram of Th-MYCN tumor cores depicting distribution of CD45-infiltrated low/high regions of interest, plotted against treatment group and duration. c Volcano plot of genes upregulated and downregulated in TEPA-treated high versus low regions of interest. False discovery rate (FDR) was adjusted using the two-tailed Benjamini–Hochberg procedure. Thresholds: p < 0.1; |log2FC | > 0.5. d Gene set enrichment analysis for TEPA-treated high-infiltrated tumor regions compared to low-infiltrated regions presented as a bar plot. e Network representation of selected pathways in (d) displaying differentially expressed genes as branches. The magnitude of change is reported as log2FC using colored nodes. Abbreviations: FC fold change, PanCK pancytokeratin.
