Fig. 4: The neuroblastoma tumor microenvironment is sensitive to copper chelation therapy and promotes neutrophil infiltration.
From: Copper chelation redirects neutrophil function to enhance anti-GD2 antibody therapy in neuroblastoma
a Experimental design and tumor processing workflow for single-cell RNA sequencing. Schematic created in BioRender. Vittorio, O. (2024). BioRender.com/j88j488. b Uniform manifold approximation and projection (UMAP) representation of integrated samples in the tumoral compartment (13,544 cells), colored by treatment group. c Violin plots of gene expression levels associated with intracellular copper levels (Mt1, Mt2) and neuroblastoma oncogene Mycn, split by treatment group. Significance was calculated using two-tailed differential expression analysis using the MAST algorithm after batch correction with p-values displayed in figure. Horizontal line indicates data median. d Gene set enrichment analysis plot for HALLMARK_MYC_TARGETS_V1 using the fgsea package. e Split UMAP representation of immune cell compartment (12,127 cells) according to treatment arm and colored by annotated immune subsets. f Bar plot of the proportion of immune cell subsets shown in (e). g Dot plot of gene expression markers used to classify the immune subsets defined in (e).
