Fig. 6: Circadian clock neuropeptidome.
A Single-cell RNA sequencing of clock neurons reveals 16 distinct clock neuron clusters (shown in a t-SNE plot) that can be reliably identified based on known markers. B, C Clock neurons express at least 12 different neuropeptides. Based on average scaled expression (red = high and grey = low). D DH44 is a new clock-related neuropeptide which is expressed in APDN3, DN1a, LNd, and LPN (arrowheads). E Proctolin is a new clock-related neuropeptide which is expressed in one DN1p (open arrowhead) and two DN2 (filled arrowheads). F AstC is expressed in DN2 in addition to other clock neurons. Double arrowheads indicate DN3 labeled by anti-AstC, filled arrowheads indicate DN2 and open arrowheads indicate DN1p. Scale bars = 50 μm for overview and 10 μm for higher magnification images. PER Period, TIM Timeless, VRI Vrille, PDF Pigment dispersing factor, DH44 Diuretic hormone 44, AstC Allatostatin-C. G Single-cell transcriptomes of NSC express receptors for clock-related neuropeptides. Based on average scaled expression (red = high and grey = low). Source data for panels A, C, and G are provided in the Source Data file.
