Fig. 7: Paracrine signaling within the clock network.

A Expression of clock-related neuropeptide receptors in different clock clusters was identified using single-cell transcriptome analysis. B t-SNE plots showing the expression of receptors in different clock clusters. Clustering is based on Fig. 6A. C Pdfr[RA]-T2A-Gal4 drives GFP expression in all clock cell types. Arrowheads indicate different types of clock neurons that express PDFR[RA]. Scale bars = 20 μm. PDF, Pigment dispersing factor, TIM Timeless. D Schematics depicting expression of receptors in different clock neurons. The schematics are based on GFP expression using T2A-Gal4 lines for different receptors (see Supplementary Data 4 for confocal images). The numbers refer to neurons expressing that receptor in one hemisphere. E Chord diagrams showing synaptic (filled boxes) and putative peptidergic paracrine connections (arrows) between clock neurons. This figure is based on synaptic connectivity in Fig. 1E, and peptide and receptor expression (following thresholding) mapping reported in Figs. 6B–F, 7A–D, Supplementary Fig. 15, and Supplementary Data 3, 4. See Supplementary Fig.16 for a detailed explanation of the filtering of putative paracrine connections. Source data for panels A, B, and E are provided in the Source Data file.