Fig. 1: Breast cancer derived EV induced cholesterol biosynthesis in liver. | Nature Communications

Fig. 1: Breast cancer derived EV induced cholesterol biosynthesis in liver.

From: Tumor-derived miR-9-5p-loaded EVs regulate cholesterol homeostasis to promote breast cancer liver metastasis in mice

Fig. 1

a Representative images for GFP signals in liver from mice xenografted MDA-MB-231/Lck-GFP cells (231/Ctrl) or MDA-MB-231/Rab27a KD/Lck-GFP cells (231/Rab27a KD) and tumor free mice. b Liver tissue collected from 231/Ctrl mice and 231/Rab27a KD mice for 50 days and tumor free mice were subjected to RNA-seq and GSEA, showing enrichment of genes related to indicated pathways (Permutation test, n = 3 mice per group). c Total cholesterol (TC) in liver and serum in indicated group (one-way ANOVA, n = 7 mice per group). d Liver sections were stained with oil red O. Scale bars, 50 μm. e Schematic of cholesterol synthesis pathway. f The qRT-PCR showing relative mRNA levels in the liver in indicated group (one-way ANOVA, n = 6 mice per group). g Western blots showing proteins related to cholesterol synthesis in the liver in indicated group. h IHC of indicated protein in liver sections. Representative images are shown. Scale bars, 100 μm. i GFP signals in liver from mice receiving tail vein injections of Lck-GFP-labeled EVs. j Liver tissue collected from mice that had received 5 weeks of i.v. injections of EVs or control mice were subjected to RNA-seq and GSEA, showing enrichment of genes set related to cholesterol biosynthesis pathway (Permutation test, n = 3 mice per group). k Total cholesterol (TC) in liver in indicated group (one-way ANOVA, n = 5 mice per group). l Left: Liver sections were stained with oil red O in NSG mice receiving tail vein injections of EVs. Scale bars, 50 μm. Right: Quantification of the lipid droplets of the liver (one-way ANOVA, n = 5 biological replicates). m Quantification of gene expression of several markers in cholesterol synthesis pathway using qRT-PCR in NSG mice receiving indicated EVs (one-way ANOVA, n = 6 mice per group). n Immunoblot analysis of liver sample collected from mice that had received MCF-10A or MDA-MB-231-derived EVs for 5 weeks. o Immunoblot analysis of proteins related to cholesterol synthesis in primary hepatocytes treated with indicated EVs, with PBS as control. Data are presented as mean ± s.d. Source data and exact P value are provided as a Source data file.

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