Fig. 3: Nucleosomes wrap tighter in euchromatin than in heterochromatin.
From: Nucleosome wrapping states encode principles of 3D genome organization
a Dot plots show the genome-wide correlations between NRI(140) of H3 and DNase I, H3K4me1, H3K4me3, H3K9me3 or H3K27me3 counted with 100 Kb bins (n = 27,268). The signal of histone modifications was normalized by H3. b Histogram shows the distribution of fragment length of histone H3 enriched DNA fragments in the peak regions of H3K4me1, H3K4me3, H3K9me3 and H3K27me3. NRS(140)s are the nucleosome wrapping score calculated within the peaks of each markers. c Dot plots show the correlations between NRI(140) of H3 and Polr2a or total RNA-seq signal within gene body regions (n = 43511). d–f Heatmaps show the distribution of H3 NRI at gene body regions (d), 2 kb regions around transcription start sites (TSSs) (e) and 2 kb regions around transcription termination sites (TTSs) (f). Genes were sorted in descending order according to Polr2a signal in the gene body regions (n = 43511). H3 NRIs calculated with 100 bp, 1 Kb, 10 Kb and 100 Kb bin resolution were shown for gene body regions. “r” indicates the Pearson correlation coefficient in a and c. Source data are provided as a Source Data file.
