Table 1 Candidate PTPN23 interactome protein list

From: PTPN23-dependent ESCRT machinery functions as a cell death checkpoint

Protein Name

Control-biotin

Control+biotin

ALIX-biotin

ALIX+biotin

PTPN23tr-biotin

PTPN23tr+biotin

PTPN23fl-biotin

PTPN23fl+biotin

ZFYVE9

0

0

0

0

3.0

7.3

2.3

10.2

ZFYVE16

7.1

2.0

0

1.2

20.5

47.8

43.9

89.2

RABEP1

0.8

0

0.3

0.3

2.4

7.4

0.3

3.3

AZI2

0

0

0

0.9

4.5

12.5

3.3

10.4

KIFC1

1.9

2.6

0.9

0.3

1.1

2.6

2.3

3.3

CC2D1A

0.3

0.0

0.6

3.4

5.9

15.9

13.4

38.7

CALCOCO2

0.3

0

0.3

1.2

1.8

6.2

1.0

2.8

GSR

1.6

1.3

4.6

1.8

2.7

6.8

1.3

3.1

  1. Identification of binding partners of PTPN23 pulled down from the cytoplasmic fraction of indicated stable cell lines by mass spectrometry analyses. The list was ranked based on the average fold change of protein enrichment in PTPN23tr and PTPN23fl-overexpressing cell lines compared to ALIX overexpressing cell line, with a cutoff value of 2. Only proteins with a fold change greater than 2 in the presence of biotin compared to its absence were included in the table.