Fig. 9: βarr2 diffusion on CCPs domains.

A Diffusion coefficient and motion values of βarr2^mNG trajectories in the absence and presence of PTH. Data is the mean ± 95% confidence intervals (C.I.) of n = 14/1093 (basal) and n = 26/1032 (PTH) cells/average trajectories of at least N = 3 experiments. **P = 0.0074 by unpaired t-test. B–D Scheme illustrating the process of calculating the clathrin-free areas. First, a binary mask was produced from the distribution of CLTA^mNG. A Voronoi tessellation diagram was generated from this binary mask. The convergence of vertices in the diagram corresponds to the center of a circle with a radius equal to the distance from a point centered on the farthest edge of a clathrin border (B). The area of the largest empty circle (LEC) and the average of all empty circles were computed (AEC) (C). Data are the mean ± S.E.M. For n = 23/933 cells/average empty circles per cell in 3 independent experiments. Scheme showing a hypothetical molecule recruited to the plasma membrane in the center of a clathrin-empty circle (D). Scheme created in https://BioRender.com. E, F Model of MSD vs time, generated from diffusion values of trajectories for PTH₁R and βarr2 in the absence of PTH (E), and PTH₁R, βarr2 in presence of PTH and PTH^TMR (F). The horizontal discontinued magenta line represents the area of the average empty circles (AEC). The solid lines show when βarr2 and PTH₁R (E, F) would reach a clathrin spot in the absence (E) or presence (F) of PTH.