Fig. 1: Altering PSEN2 expression gives rise to distinct Aβ profiles. | Nature Communications

Fig. 1: Altering PSEN2 expression gives rise to distinct Aβ profiles.

From: Altered expression of Presenilin2 impacts endolysosomal homeostasis and synapse function in Alzheimer’s disease-relevant brain circuits

Fig. 1

a Western blot of neuronal lysates collected over time and probed for Aβ, PSEN2-CTF and PSEN1-NTF. b, c Quantification of (a) for intracellular and secreted Aβ shown as a fold change compared to APPKI. d–g MSD ELISA of Aβ species from neuronal lysates (d, e) or conditioned medium (f, g). d, f show the different Aβ peptides compared to total. e, g represent Aβ ratios indicating γ-secretase processivity. For (b–g) samples were collected from 3 different cultures (N = 3) where 2 dishes were collected from each culture (n = 6). h–j MSD ELISA of Aβ species from mouse brain lysate. Statistical analysis was performed using a two-way ANOVA with Tukey’s correction for multiple testing compared to APPKI, p-values and N-numbers are indicated in the figure. All graphs are represented as mean ± SEM.

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