Fig. 3: PSEN2 deficiency and FAD-linked mutant PSEN2 lead to working memory deficits.

For all the behavioral tests the cohorts consist of wild-type N = 14, APP NL G F N = 15, APPxPSEN2KO N = 17, APPxFADPSEN2 N = 16 where N represents individual female mice. a Total laser crossings of wild-type, APPKI, APPxPSEN2KO and APPxFADPSEN2 mice summed per 30 min and represented over time. Gray square indicates nocturnal hours with increased activity. b Area under the curve calculated based on graph represented in A. Statistical analysis was done using one-way ANOVA with Dunnet’s correction for multiple testing comparing to APPKI. c Schematic of the Morris water maze paradigm with 10 training days and a probe trial on day 6 and day 11. d Average distance swam to find the platform per genotype per day over the total training period. e Average time spent in the target quadrant versus other quadrants. For time spent in target quadrant, a two-tailed t-test was used comparing average to 25% indicating presence in quadrant is higher than chance level. For comparison of time spent in other quadrants versus target, one-way ANOVA was performed with Dunnett’s multiple comparisons test. f Schematic of the matching to platform paradigm with 5 total testing days. g–j Average distance swam over the different daily trials averaged over the total testing days. Subsequent swims were compared to swim 1 using a one-way ANOVA with Tukey’s corrections for multiple testing. All graphs are mean ± SEM, with p- and n-values indicated.