Fig. 3: CUL5 knockout (KO) CD19 chimeric antigen receptor (CAR) T cells upregulate the Janus kinase/signal transducers and activators of the transcription (JAK/STAT) pathway. | Nature Communications

Fig. 3: CUL5 knockout (KO) CD19 chimeric antigen receptor (CAR) T cells upregulate the Janus kinase/signal transducers and activators of the transcription (JAK/STAT) pathway.

From: Cullin-5 deficiency promotes chimeric antigen receptor T cell effector functions potentially via the modulation of JAK/STAT signaling pathway

Fig. 3

a Numbers of differentially expressed genes (DEGs) in control (Ctrl) and two different sgRNA-modified CUL5 KO-CD19 CAR T cells (Ctrl: n = 4, sgRNA#4690: n = 4, and sgRNA#8377: n = 3). b Venn diagram of DEGs in CUL5 KO-CD19 CAR T cells prepared using two different sgRNAs. c Heatmap displaying the significantly upregulated and downregulated genes in comparisons between CUL5KO-CD19CAR T cells treated with sgRNA#4690 and Ctrl CAR T cells. Each column represents the respective donor’s result. d Over-representation analysis (hallmark gene sets) of upregulated genes in CUL5 KO-CD19 CAR T cells versus Ctrl sgRNA-transduced CD19 CAR T cells. The p-values were all adjusted by Benjamini–Hochberg false discovery rate (FDR) procedure. –log10 (adjusted p-value) is shown.

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