Fig. 1: ATXN1L deficiency specifically reduces MZP and MZB cell populations.

a Flow cytometry of B-1a (CD19⁺CD5⁺CD43⁺) and B-1b (CD19⁺CD5^-CD43⁺) cells in the peritoneal cavities and spleens of Cd19-Cre and Atxn1l^f/f;Cd19-Cre mice. For peritoneal cavity analysis, N = 5 per both mice. For spleen analysis, N = 7 for Cd19-Cre and N = 5 for Atxn1l^f/f;Cd19-Cre mice. Flow cytometry of FOB and MZB cells (b), MZP cells (c), and T1B and T2B cells (d) in the spleens of Cd19-Cre and Atxn1l^f/f;Cd19-Cre mice. FOB (CD19⁺CD93^-CD21^intCD23⁺) and MZB (CD19⁺CD93^-CD21^highCD23^lo) cells were initially gated as CD93^- (AA4.1). T1B (CD19⁺CD93⁺B220⁺CD21^loCD23^-IgM⁺), T2B (CD19⁺CD93⁺B220⁺CD21^midCD23⁺IgM⁺), and MZP (CD19⁺CD93⁺CD21^high) cells were gated as CD93⁺ (AA4.1). N = 7 for Cd19-Cre and N = 5 for Atxn1l^f/f;Cd19-Cre. e Western blotting to detect the levels of CIC, ATXN1, and ATXN1L in FOB, MZB, and B-1a cells. FOB and MZB cells were prepared from the spleens of C57BL/6 mice, whereas B-1a cells were isolated from the peritoneal cavities of the same mice. Data represent 2–3 independent experiments. Statistics: two-tailed Student’s t-test (a–d). Bar graphs present the data as mean ± S.D. Ctrl: Cd19-Cre and A1L cKO: Atxn1l^f/f;Cd19-Cre. MZP marginal zone progenitor cells, MZB marginal zone B cells, and FOB follicular B cells. Source data are provided as a Source Data file.