Fig. 4: BCR signaling is attenuated in Cic-null FOB cells. | Nature Communications

Fig. 4: BCR signaling is attenuated in Cic-null FOB cells.

From: The capicua-ataxin-1-like complex regulates Notch-driven marginal zone B cell development and sepsis progression

Fig. 4

Analysis of anti-IgM-stimulated and tonic BCR signaling in Cic-null (a) and Atxn1l-null (b) FOB cells. Phosphorylated-ERK (pERK), phosphorylated-BTK (pBTK), phosphorylated-BLNK (pBLNK), and phosphorylated-AKT (pAKT) levels were determined using flow cytometry and presented as MFI. For the BCR signaling analysis in control and Cic-null FOB cells, N = 6 per group (a). For the analysis of pERK and pBTK levels in control and Atxn1l-null FOB cells stimulated with anti-IgM, N = 4 per group (b). For the analysis of pBLNK levels in control and Atxn1l-null FOB cells stimulated with anti-IgM, N = 3 per group (b). For the analysis of pAKT levels in control and Atxn1l-null FOB cells stimulated with anti-IgM, N = 4 for Cd19-Cre and N = 3 for Atxn1lf/f;Cd19-Cre (b). For the tonic BCR signaling analysis in control and Atxn1l-null FOB cells, N = 8 for Cd19-Cre and N = 6 for Atxn1lf/f;Cd19-Cre (b). Data represent 2–3 independent experiments. Statistics: two-tailed Student’s t-test (a, b). Bar graphs present data as mean ± S.D. Ctrl: Cd19-Cre, Cic cKO: Cicf/f;Cd19-Cre, and A1L cKO: Atxn1lf/f;Cd19-Cre. BCR B cell receptor, MFI mean fluorescence intensity, and FOB, follicular B cells. Source data are provided as a Source Data file.

Back to article page