Fig. 6: Etv4 is a CIC target gene that suppresses Notch signaling and MZB cell development. | Nature Communications

Fig. 6: Etv4 is a CIC target gene that suppresses Notch signaling and MZB cell development.

From: The capicua-ataxin-1-like complex regulates Notch-driven marginal zone B cell development and sepsis progression

Fig. 6

RT-qPCR analysis of Etv4 and Etv5 levels in MZB cells from Cicf/f;Cd19-Cre (a) and Atxn1lf/f;Cd19-Cre (b) mice. N = 3 per group. c Flow cytometry of NOTCH1 and NOTCH2 levels in MZB cells infected with either a control (NC) or ETV4-expressing (ETV4) retrovirus. N = 6 per group. d ChIP-qPCR analysis of the Notch1 and Notch2 promoter regions containing ETV4 binding motifs. B-2 cells isolated from the spleens of Cicf/f;Cd19-Cre mice were subjected to ChIP using either IgG or an anti-ETV4 antibody. N = 3 per group. Flow cytometry of splenic FOB and MZB cells (e), MZP cells (f), and T1B and T2B cells (g) in Cd19-Cre, Cicf/f;Cd19-Cre, and Etv4−/−;Cicf/f;Cd19-Cre mice. N = 7 for Cd19-Cre, N = 8 for Cicf/f;Cd19-Cre, and N = 5 for Etv4−/−;Cicf/f;Cd19-Cre. Ctrl: Cd19-Cre, cKO: Cicf/f;Cd19-Cre, and DKO: Etv4−/−;Cicf/f;Cd19-Cre. Flow cytometry of splenic FOB and MZB cells (h) and MZP cells (i) in Cd19-Cre, Atxn1lf/f;Cd19-Cre, and Etv4−/−;Atxn1lf/f;Cd19-Cre mice. N = 8 for Cd19-Cre, N = 6 for Atxn1lf/f;Cd19-Cre, and N = 3 for Etv4−/−;Atxn1lf/f;Cd19-Cre. Ctrl: Cd19-Cre, cKO: Atxn1lf/f;Cd19-Cre, and DKO: Etv4−/−;Atxn1lf/f;Cd19-Cre. j Surface expression levels of NOTCH1, NOTCH2, and CD21 in MZB and MZP cells from Cd19-Cre, Cicf/f;Cd19-Cre, and Etv4−/−;Cicf/f;Cd19-Cre mice. N = 5 per group. k Surface expression levels of NOTCH1, NOTCH2, and CD21 in MZB and MZP cells from Cd19-Cre, Atxn1lf/f;Cd19-Cre, and Etv4−/−;Atxn1lf/f;Cd19-Cre mice. N = 5 for Cd19-Cre and Atxn1lf/f;Cd19-Cre, and N = 3 for Etv4−/−;Atxn1lf/f;Cd19-Cre. The MFIs for NOTCH1, NOTCH2, and CD21 were determined using flow cytometry. Data represent 2–3 independent experiments. Statistics: two-tailed Student’s t-test (a–d) and one-way ANOVA with Tukey’s multiple comparisons test (e–k). Bar graphs present data as mean ± S.D. RT-qPCR quantitative real-time polymerase chain reaction, FOB follicular B cells, MZB marginal zone B cells, MZP marginal zone progenitor cells, and MFI mean fluorescence intensity. Source data are provided as a Source Data file.

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