Fig. 7: Restoration of IgM production and sepsis progression in Etv4−/−;Cicf/f;Cd19-Cre mice.
a, b Cd19-Cre, Cicf/f;Cd19-Cre, and Etv4−/−;Cicf/f;Cd19-Cre mice were immunized with NP-Ficoll for 7 days. a Flow cytometry of splenic plasma cells. N = 5 for Cd19-Cre and Cicf/f;Cd19-Cre, and N = 4 for Etv4−/−;Cicf/f;Cd19-Cre. b Serum NP-specific IgM and IgG levels determined using ELISA. N = 5 for Cd19-Cre and N = 4 for Cicf/f;Cd19-Cre and Etv4−/−;Cicf/f;Cd19-Cre. c–e Cd19-Cre, Cicf/f;Cd19-Cre, and Etv4−/−;Cd19-Cre mice were immunized with OVA in alum for 7 days. Flow cytometry of splenic GCB cells (c) and plasma cells (d). N = 7 for Cd19-Cre, N = 8 for Cicf/f;Cd19-Cre, and N = 6 for Etv4−/−;Cicf/f;Cd19-Cre. e Serum OVA-specific IgM and IgG levels determined using ELISA. N = 7 for Cd19-Cre, N = 8 for Cicf/f;Cd19-Cre, and N = 5 for Etv4−/−;Cicf/f;Cd19-Cre. f–h Female Cd19-Cre, Cicf/f;Cd19-Cre, and Etv4−/−;Cicf/f;Cd19-Cre mice were injected intravenously with LPS. f Survival rate of LPS-treated mice. Mortality was monitored every 24 h. N = 7 for Cd19-Cre and Etv4−/−;Cicf/f;Cd19-Cre, and N = 10 for Cicf/f;Cd19-Cre. g Serum IL-6 and IL-10 levels determined using ELISA. h Histology of immune cell infiltration into lung tissue. Arrows indicate immune cell-infiltrated regions. Scale bar: 100 µm. Serum samples and lung tissues were collected from the mice 12 h after LPS injection. N = 5 per group. Data represent 2–3 independent experiments. Statistics: one-way ANOVA with Tukey’s multiple comparisons test (a–e, g–h) and Log-rank (Mantel-Cox) test (f). The bar graph presents data as mean ± S.D. Ctrl: Cd19-Cre, cKO: Cicf/f;Cd19-Cre, and DKO: Etv4−/−;Cicf/f;Cd19-Cre. ELISA enzyme-linked immunosorbent assay, OVA ovalbumin, and GCB germinal center B cell. Source data are provided as a Source Data file.
