Fig. 1: IONP-COOH⁻²¹ inhibited leukaemia progression by indirectly killing.

A The apoptosis analysis of BCR-nup cells incubated with IONP-COOH⁻²¹ at 200 μg mL⁻¹ for 24 h (n = 3 technical replicates; the experiment was independently repeated on three different days), apoptosis ratio (%) = Q1 + Q2. B Number of MOLM-13 cells after co-incubation with IONP-COOH⁻²¹ for 24 h at different iron concentrations (n = 3 technical replicates; the experiment was independently repeated on three different days), data of (A, B) are represented as mean ± SD. C–E, AML mice were injected (IV) with saline or IONP-COOH⁻²¹ 6 mg kg⁻¹ on day 11, and tumour burden was analyzed on day 12 (n  =  5 mice). The leukaemic cells were identified by human CD45⁺ expression. Experimental scheme (F) and Kaplan-Meier survival curves (G) for the effect of IONP-COOH⁻²¹ on overall survival (n = 10 mice/group), created in BioRender. https://BioRender.com/o33l748. Unpaired two-tailed t-tests were used in (A–E). Survival curves were compared using a log-rank Mantel-Cox test. Source data are provided as a Source Data file.