Fig. 2: IONP-COOH⁻²¹ suppressed myeloid leukaemia progression in a complement-dependent manner.

C57BL/6N AML mice were treated with saline (n = 5 mice) or IONP-COOH⁻²¹ at 6 mg kg⁻¹ (n = 7 mice) on days 3 and 8. The percentage (A) and absolute cell counts (B) of leukaemia cells were analyzed on day 10. C Number of tumour cells after depleted macrophages. n = 4 mice, liposome control; n = 8 mice, clodronate group and IONP-COOH⁻²¹-treated group. D Red, macrophages, F4/80-PE; Green, leukaemia cells, CFSE; White, macrophages engulfed leukaemia cells. Scale bar, 50 µm. n = 9 technical replicates, saline and IONP-COOH⁻²¹-treated WT groups; n = 12 technical replicates, C3KO group. Primary cells were isolated from five mice, and the experiment was independently repeated on three different days. E Counts of leukaemic cell. Percentage of Granzyme B and IFN-γ in CD8⁺, CD4⁺ T (F, G) and NK cells (H). E–H. n = 10 mice, saline- WT; n = 11 mice, IONP-COOH⁻²¹- WT; n = 5 mice, C3 knockout. All data are presented as mean ± SD. A two-tailed t-test was used in (B). One-way ANOVA with multiple comparison adjustments was applied in others. Source data are provided as a Source Data file.