Fig. 3: Physicochemical characterisation and antileukaemia effects of IONPs.
A XPS analysis of high-resolution O1s spectrum of IONP-OH and IONP-COOH and high-resolution N1s spectrum of IONP-NH2. The XPS survey spectra of the corresponding nanomaterials were presented in the inset. B The hydrodynamic size and zeta potential of IONPs were assessed both before (blue) and after (red) incubation with human plasma. The IONPs were incubated with human plasma and washed three times. Each experiment was repeated twice using samples obtained from three different donors, with three technical replicates performed for each measurement. C NSG mice were inoculated intravenously (IV) with 5 ×105 MOLM-13 cells to establish an AML mouse model. The mice were injected (IV) with saline or IONPs 6 mg kg−1 on day 11 and the tumour burden was analyzed by flow cytometry on day 12 (n = 3 mice/group). The absolute numbers of AML cells were calculated by multiplying the frequency of human CD45+ cells by the total concentration of splenic cells or bone marrow cells from the femur. Bars represent the mean, and error bars represent SD. One-way ANOVA with multiple comparison adjustments was applied in (C). D NSG mice treated with saline or 6 mg kg−1 of IONPs by (n = 10 mice/group), survival curves were analyzed using the Kaplan-Meier method. Source data are provided as a Source Data file.
