Fig. 4: IONPs with different functional groups activated the complement system through distinct complement pathways. | Nature Communications

Fig. 4: IONPs with different functional groups activated the complement system through distinct complement pathways.

From: Specific surface-modified iron oxide nanoparticles trigger complement-dependent innate and adaptive antileukaemia immunity

Fig. 4

A 3D PCA for protein. B–C Protein corona was identified by LC-MS. D Scheme of complement pathways (Created in BioRender. https://BioRender.com/b64n273). E, Bioinformatic pathway analysis for top 20 proteins based on KEGG pathway database. F Quantitative assay of C3b and properdin number per IONP. The dots represent individual data points corresponding to plasma samples collected from three different individuals (n = 3 healthy donor samples, biological replicates). The experiment was independently repeated three times. Data were evaluated by two-way ANOVA with multiple comparison adjustments. G Western blot of Bb/C3b after incubation with AML plasma. Samples were derived from the same experiment batch but different gels for Bb, and another for C3b were processed in parallel. The experiment was repeated three times independently with similar results. H ELISA detection of C1s/C1-INH and MASP/C1-INH complexes. The dots represent individual data points corresponding to plasma samples collected from different individuals (n = 6 healthy donor samples for C1s/C1-INH, n = 3 healthy donor samples for MASP/C1-INH, all representing biological replicates). The experiment was independently repeated twice. Data were evaluated by one-way ANOVA with multiple comparison adjustments. I Curves of the C3b/IgG and IONPs by biolayer interferometry. Source data are provided as a Source Data file.

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