Fig. 6: Surface residues influenced IONPs’ homoeostasis in vivo. | Nature Communications

Fig. 6: Surface residues influenced IONPs’ homoeostasis in vivo.

From: Specific surface-modified iron oxide nanoparticles trigger complement-dependent innate and adaptive antileukaemia immunity

Fig. 6

A Schematic illustration for in vivo immune system study on C57BL/6N mice given a single dose of 10 mg Fe kg−1 IONPs injection (Created in BioRender. https://BioRender.com/h38h965). B Visualisation of the major splenocytes using tSNE plots after different IONPs treatments. C The number of peripheral WBCs changes following IONP administration, data are presented as mean ± SD, with n = 3 mice, experiment was repeated twice independently with similar results. Data were evaluated by two-way ANOVA with multiple comparison adjustments. The flow cytometry gate strategy is shown in Supplementary Fig.12. D Representative histological images for tissue sections with hematoxylin and eosin staining (H&E) to evaluate biosafety in vivo. The organs were stained with H&E 24 h after saline or IONP injection. H&E staining showed structural derangement in the spleen (black arrows). Scale bar, 100 µm.

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