Fig. 2: Cryo-EM 3D variability analysis presents constrained flexibility of the ADGRL3 GAIN domain. | Nature Communications

Fig. 2: Cryo-EM 3D variability analysis presents constrained flexibility of the ADGRL3 GAIN domain.

From: Conformational coupling between extracellular and transmembrane domains modulates holo-adhesion GPCR function

Fig. 2

a–c Side and top views of representative low-resolution 3D models from 3D variability analysis show the degree of HormR/GAIN domain movement in relation to the detergent micelle. Models from a the first frame of 3D variability analysis, showing the most vertical orientation of the GAIN domain seen within the dataset; b The middle frame of 3D variability analysis, presenting the intermediate position of the GAIN domain; c The last frame of 3D variability analysis, showing the most horizontal orientation of the GAIN domain. The red triangles on each panel depict the degree of the angle between the bottom of the GAIN domain and the plane of the micelle. The red areas highlighted on the top-view projections show the increasing access of the transmembrane region to the extracellular space with the GAIN domain pulling away from the central axis. d Superimposition of the most distinct frames (a, b) showing the distances and angle between two GAIN conformations. Structures of HormR/GAIN domains of ADGRL3 in complex with LK3 are placed in the maps by rigid body fitting.

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