Fig. 4: Compromised cerebellar-dependent plasticity in the paraflocculus/flocculus in 22q11DS mice. | Nature Communications

Fig. 4: Compromised cerebellar-dependent plasticity in the paraflocculus/flocculus in 22q11DS mice.

From: Tbx1 haploinsufficiency leads to local skull deformity, paraflocculus and flocculus dysplasia, and motor-learning deficit in 22q11.2 deletion syndrome

Fig. 4

a Schematic of the recording and stimulation configuration for long-term depression (LTD) induction. Purkinje cells (PCs) were targeted for whole-cell recording while parallel fiber (Pf) and climbing fiber (Cf) inputs were stimulated. b Normalized excitatory postsynaptic potential amplitude (EPSP amp.) shows the LTD time course before and after a 10-min baseline in WT (black) and Df(16)1/+ (red) PCs; LTD was induced by conjunctive Pf and Cf stimulation. Example voltage traces from individual cells (upper) show EPSPs induced by Pf stimulation before (left) and after (right) LTD induction in WT and Df(16)1/+ PCs. The dotted lines show the amplitude of the baseline response. c Average LTD amplitude in WT (n = 7 cells, 4 mice) and Df(16)1/+ (n = 8 cells, 5 mice) PCs. Two-tailed Student’s t-test (Welch’s correction): t8.66 = 2.77, *p = 0.023. d Normalized EPSP amplitude shows the LTD time course, before and after the 10-min baseline in WT (black) and Tbx1+/– (green) PCs. Example voltage traces from individual cells (upper) show EPSPs induced by Pf stimulation before (left) and after (right) LTD induction in WT and Tbx1+/– PCs. The dotted lines show the amplitude of the baseline response. e Average LTD amplitude in WT (n = 8 cells, 5 mice) and Tbx1+/– (n = 9 cells, 6 mice) PCs. Two-tailed Student’s t-test (Welch’s correction): t8.24 = 2.41, *p = 0.042. f Schematic of the recording and stimulation configuration for long-term potentiation (LTP) induction. PCs were targeted for whole-cell recording while Pf inputs were stimulated. g Normalized LTP time course before and after 5-min baseline in WT and Df(16)1/+ PCs, showing LTP induction by tetanic PF stimulation. h Average LTP amplitude in WT (n = 7 cells, 5 mice) and Df1+/– (n = 10 cells, 5 mice) PCs. Two-tailed Student’s t-test (Welch’s correction): t11.33 = 0.04, p = 0.968. i Normalized the LTP time course before and after 5-min baseline in WT and Tbx1+/– PCs, showing LTP induction by tetanic PF stimulation. j Average LTP amplitude in WT (n = 10 cells, 8 mice) and Tbx1+/– (n = 7 cells, 5 mice) PCs. Two-tailed Student’s t-test (Welch’s correction): t9.86 = 0.94, p = 0.37. Data are presented as the mean ± SEM. n.s. not significant. Source data are provided as a Source Data file.

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