Fig. 3: [¹⁸F]FSPG PET can differentiate NRF2-high from NRF2-low tumours when grown orthotopically in the lungs of mice.

a Single slice CT axial images (top) and ex vivo H&E images (middle; scale bar = 5 mm) of lungs containing H1299 or H460 tumours, with xCT staining of corresponding tumours (bottom; scale bar = 50 µm). b Representative in vivo [¹⁸F]FSPG PET/CT maximum intensity projections (MIPs; top) and axial single-slice PET/CT (bottom) of mice bearing H1299 or H460 orthotopic lung tumours. Dashed lines represent the tumour outline. P pancreas, K kidney, B bladder. c Quantified [¹⁸F]FSPG retention in individual tumour lesions from orthotopic tumour-bearing mice. Comparison was made using an unpaired two-tailed Student’s t-test. n = 7-21 lesions from 3 to 9 mice per cohort. The median value (center line), lower quartile and upper quartile (box edges) and maximum and minimum value whiskers are indicated in the boxplot. d Representative western blot for xCT and NRF2 expression in H1299 and H460 orthotopically grown tumours from n = 4 mice per group. Actin was used as a loading control. For (c, d), source data are provided as a Source Data file.