Fig. 4: [¹⁸F]FSPG retention is increased in Nrf2 mutant mice.

a Scheme depicting tumour formation in KP and KPN mice. KP mice conditionally express oncogenic Kras and have loss of p53 function. KPN mice conditionally express oncogenic Kras, have loss of p53 function and express a mutant Nrf2, which increases Nrf2 protein levels. b CT MIP representing individual 3D tumour regions of interest. c Total tumour volumes in the lungs of KP and KPN mice. Data are presented as mean ± SD from n = 5-6 mice. d Representative coronal [¹⁸F]FSPG PET/CT images of 40–60 min summed activity in KP and KPN tumour-bearing mice. Dashed white lines indicate the lung. B bladder, P pancreas. e Violin plots of [¹⁸F]FSPG tumour retention from individual lesions. Dashed lines represent the median and the upper and lower quartiles. n = 63–105 lesions from 5–6 mice per cohort. f Nrf2 expression in KP and KPN tumour lesions. Actin was used as a loading control. g Representative IHC staining of xCT from lesions taken from KP and KPN mice (scale bars = 20 μM). h H-scores for xCT IHC staining. Data are presented as mean ± SD from n = 10 mice. i Heatmap depicting the H-scores for xCT IHC staining by tumour grade. AAH adenomatous atypical hyperplasia, BH bronchiolar hyperplasia. All comparisons were made using an unpaired two-tailed Student’s t-test. a created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license, https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en. For (c, e, f, h), source data are provided as a Source Data file.