Fig. 2: Cellular uptake, retention, and mitochondria targeting. | Nature Communications

Fig. 2: Cellular uptake, retention, and mitochondria targeting.

From: MMP-2-triggered, mitochondria-targeted PROTAC-PDT therapy of breast cancer and brain metastases inhibition

Fig. 2

a, b Cellular uptake assay of different formulations by fluorescence microscope (scale bar = 100 μm). c, d Cellular uptake assay of different formulations by flow cytometry (n = 3 independent cell lines). e Confocal images of 4T1 MSCs incubated with free Ce6, CPD, CFPD, and CFMPD for 12 h (scale bar = 100 μm). f Quantitative analyses of tumor spheroid sections at 100 μm. g Mitochondrial localization analyses of different formulations in 4T1 cells (scale bar = 20 μm). h TEM image of cells treated with CFMPD (scale bar = 1 μm). Red arrows indicate mitochondrial cristae. All data are presented as mean ± SD. Two-tailed Student’s t test and One-way analysis of variance (ANOVA) with a Tukey post hoc test were used for the statistical comparison between the two groups and among multiple groups, respectively. A significant difference was considered when the p value was <0.05. Source data are provided as a Source Data file.

Back to article page