Fig. 2: Olig2 is a regulator of stem cell proliferation.
From: OLIG2 mediates a rare targetable stem cell fate transition in sonic hedgehog medulloblastoma
a HOMER motif analysis of uniquely accessible regions in the cycling (MKI67+) and non-cycling (MKI67−) fractions of the stem (SOX2+) cells; n = 2 biologically independent samples; motif enrichment is calculated using cumulative binomial distributions. b Overlap of OLIG2 binding peaks in mNSCs (data obtained from Mateo et al.53) with ATACseq peaks of SOX2+/− (top pie chart) as well as SOX2+/MKI67+ and SOX2+/MKI67– (bottom pie chart) tumour fractions. c Gene Ontology Biological Process (GO BP) analysis of genes associated with the overlaps in (b). d, e Immunocytochemistry of Ptch1+/−;Trp53−/− mouse tumour cells (d), quantification of EdU+ cells after a 1-hour pulse averaged across three biological replicates (e); n = 3, error bars denote mean ± SEM; two-tailed unpaired t-test; ns p ≥ 0.05, ****p < 0.0001; p > 0.9999 (EdU+ vs. OLIG2+), p < 0.0001 (EdU+ vs. EdU+/OLIG2+), p < 0.0001 (OLIG2+ vs. EdU+/OLIG2+). f Phase object confluence of adherently grown Ptch1+/−;Trp53−/− CRISPR-Cas9 control or Olig2-KO mouse tumour cells measured across 14 days using live cell imaging; data shown are representative of three independent experiments; error bars denote mean ± SEM; two-tailed unpaired t-test performed at mid-exponential phase time point (144 hours); p = 0.0021 (control vs. Olig2-KO). g Limiting dilution analysis (LDA) performed on Ptch1+/−;Trp53−/− CRISPR-Cas9 control or Olig2-KO cells; n = 3 biological replicates; individual replicates were assessed by goodness of fit testing using extreme limiting dilution analysis (ELDA) software (see Methods); error bars denote mean ± SEM; unpaired t-test; two-tailed p-value; p = 0.0182. h Tumour volumes in NOD-scid-gamma (NSG) mice subcutaneously engrafted in both flanks with either Ptch1+/−;Trp53−/− CRISPR-Cas9 control or Olig2-KO cells. n = 4 mice (8 flanks) for control and n = 3 (6 flanks) for Olig2-KO condition; error bars denote mean ± SEM; chi-square test; p = 0.0157; f–h: *p < 0.05, **p < 0.01. Source data are provided as a Source Data file.
