Fig. 2: CR fusions disrupt myeloid differentiation of human HSPCs.

a Colony-forming assays of human CD34⁺ cells transduced with the indicated genes in the H4435 methylcellulose medium. n = 3 independent donors in n = 3 independent experiments (Vec group) and in n = 4 independent experiments (CRL and CRS groups). b Serial colony-forming assays of the cells used in a. n = 3 independent donors in n = 3 independent experiments. c The growth curves of human CD34⁺ cells ectopically expressing with the indicated genes in myeloid differentiation medium. Statistical analysis was performed by unpaired two-tailed Student’s t-test. d Flow cytometry quantification of myeloid precursors (CD34⁺CD33⁺) and myeloid mature cells (CD66b⁺CD33⁺) in myeloid differentiation medium on day 14. e Statistical results and representative images of morphologic myelocytes and myeloid mature cells. Scale bars, 5 μm. n = 2 independent donors in n = 4 independent experiments (c–e). f–h The frequencies of myeloid cells (f), HSPCs (g), and B cells (h) in engrafted GFP⁺ cells in xenograft models. CD33⁺, myeloid cells (Vec, n = 5 mice; CRL, n = 8 mice, CRS, n = 7 mice); CD34⁺CD33⁺, myeloid precursors; CD33⁺CD66b⁺, granulocytes (n = 4 mice/group); CD34⁺CD38^‒, primitive stem cell populations; CD34⁺CD38⁺; committed progenitors (n = 5 mice/group); CD19⁺, B cells (Vec, n = 5 mice; CRL, n = 8 mice, CRS, n = 7 mice). All the data are shown as the mean ± SD. Statistical analysis was performed by two-tailed one-way ANOVA with Dunnett’s multiple comparisons test between the CR group and Vec group (a, b, d–h). See also Supplementary Fig. 3. Source data are provided as a Source Data file.