Fig. 4: CR fusion alters target gene profiles by increasing chromatin occupancy.

a–b Heatmaps (a) and profiles (b) of CUT&Tag-seq signal intensities displaying loci clusters of overlapping peaks for CRL and CRS in human CD34⁺ cells. c Genome-wide distribution of overlapping peaks for CRL and CRS. d Heatmaps presenting the common differentially expressed genes of CRL and CRS identified by RNA-seq in human CD34⁺ cells. e Venn diagram showing that overlapping genes were targets for CR fusions. f Enrichment analysis of activated and repressed genes using the gene sets specifically expressed in myeloid cells with 10 differentiation stages. PMs, promyelocytes; MYs, myelocytes; MMs, metamyelocytes; BCs, band cells; PMNs, polymorphonuclear cells. g Top enriched signatures in activated or repressed targets in human CD34⁺ cells expressing CRL or CRS. h Pre-ranked GSEA to examine the specificity of CR activated and repressed target genes using RNA-seq data from CR-AML and non-CR AML patients. The statistical significance of enrichment was determined by the two-tailed hypergeometric test (f, g). See also Supplementary Fig. 5. Source data are provided as a Source Data file.