Fig. 4: CT-179 induces apoptosis in MB explant organoids.
From: Suppressing recurrence in Sonic Hedgehog subgroup medulloblastoma using the OLIG2 inhibitor CT-179
A H&E of untreated MB organoids R403, R901 and R902. SHH-activated MB organoids R902 are immunoreactive for GAB1. CD31 staining shows endothelial cells. KI67 staining shows proliferating cells. SOX2, which identified both glial cells and tumor stem cells, is highly expressed in SHH-MB organoid R902. Focal OLIG2 staining is positive in R902 organoid. B OLIG2 expression in patient tissues R901 and R902 (means ± SD, n = 2). Only R902 patient specimen is positive for OLIG2. C SHH-MB organoid R902 was treated for CT-179 (1 μM) or RT (2 Gy) alone or in combination for 48 hours. IHC and IF show cC3, KI67 and SOX2 (means ± SD, n = 3, The p values by two-sided Student’s t-test). D Kaplan-Meier survival curves of Daoy-luci mice, compared by Log-Rank test (≥6 per group). MS: median survival. E Luminescence signal quantitation of Daoy-luci brain tumors (means ± SD, n = 9 for control, CT-179 and RT, n = 10 CT-179 + RT, p values by 1-way ANOVA and Tukey’s multiple comparison test). F Luminescence signal quantitation of Daoy-luci spinal metastases (means ± SD, n = 9 for control, CT-179 and RT, n = 10 CT-179 + RT, p values by 1-way ANOVA and Tukey’s multiple comparison test)). G Kaplan-Meier survival curves of Med-813-luci mice (≥6 per group) treated with indicated therapies. MS: median survival. The p values were determined by the Log-Rank test. C dots indicate individual biological replicates and columns indicate means. Source data are provided as Supplementary Data 4 Source Data file.
