Fig. 8: Blockade of LTβR-nonclassical NFΚB signaling reduces CD4 Tregs in dLNs and suppresses melanoma lymphatic metastases.
From: Regulatory T cells crosstalk with tumor cells and endothelium through lymphotoxin signaling
a, b Same as in Fig. 7, B16F10 melanoma-bearing C57BL/6 mice treated with a peritumoral injection of 10 nmol/tumor of nciLT, ciLT, or CP for 5 days. At day 13, dLNs (n = 4) analyzed for CD4, CD8, CD25+, or CD25− Foxp3+ CD4 Tregs by flow cytometry (a). The gating strategy is shown. Draining LNs (n = 5 per group) at day 20 assessed for tumor metastasis (Melan-A), Tregs (Foxp3), and lymphangiogenesis (Lyve-1) by immunohistochemistry (b). Representative image with scale bar: 500 μm. a, b. Representative of two independent experiments. Mean ± SEM. **p = 0.0015, ****p < 0.0001 by one-way ANOVA. Source data are provided as a Source Data file.
