Fig. 2: The hyperthermia induced gene expression by engineered bacteria. | Nature Communications

Fig. 2: The hyperthermia induced gene expression by engineered bacteria.

From: Thermal-responsive activation of engineered bacteria to trigger antitumor immunity post microwave ablation therapy

Fig. 2

A The scheme of the expression of IL-15&IL-15Rα or mCherry protein in engineered bacteria under hyperthermia, the figure was created in BioRender. Yujie (2024) https://BioRender.com/x95f139. Fluorescence images (B) and quantitative analysis (C) of mCherry fluorescence in mCherry@VNP under different temperature conditions (n = 3 samples, p value was calculated by the two-tailed Student’s t test). D The growth activity of engineered bacteria after treatment under different temperatures, as indicated by OD600 (n = 3 samples). Scheme (E, the figure was created in BioRender. Yujie (2024) https://BioRender.com/k68x589), fluorescence image (F), and quantitative analysis (G) of mCherry in mCherry@VNP after heat induction for different durations (n = 3 samples, p values were calculated by one-way ANOVA with Tukey’s multiple comparisons). Scheme (H, the figure was created in BioRender. Yujie (2024) https://BioRender.com/r04v960), fluorescence image (I), and quantitative analysis (J) of mCherry in mCherry@VNP at different time points after 15 min of heat induction (n = 3 samples). Scheme (K, the figure was created in BioRender. Yujie (2024) https://BioRender.com/k18d759) and western blot images (L) of IL-15&IL-15Rα protein in bacterial pellets and induced supernatants of 15&15R@VNP under different temperature conditions, this experiment was repeated for three times independently with similar results. Data in C, D, G, and J were presented as mean ± SD. Sup supernatant. Source data are provided as a Source Data file.

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