Fig. 1: Absence of enzymatically active TYK2 results in less severe candidiasis.

a WT, Tyk2^-/-, and Tyk2^K923E mice were intradermally (i.d) injected in the back skin with 1 × 10⁸ CFU of C. albicans or PBS (control). Fungal load in the skin (b) and in the kidneys (c) was measured on days 2 and 4 post-infection (p.i). For each time-point, pooled data from 2 independent experiments are shown, and median values are given; n: biological replicates; the dotted line indicates the assay detection limit (b, c). Skin day 2: n = 10 (WT), n = 12 (Tyk2^-/-) and n = 9 (Tyk2^K923E); skin day 4: n = 11 (WT, Tyk2^-/-) and n = 12 (Tyk2^K923E) (b); kidney day 2: n = 10 (WT, Tyk2^K923E) and n = 12 (Tyk2^-/-); kidney day 4: n = 11/genotype (c). d Blood cell composition on day 4 p.i was determined with a Vet ABC analyzer. The percentage of granulocytes and lymphocytes out of total white blood cells (WBC) is shown. Pooled data from 2 independent experiments are shown. Mean values ± SEM are given; PBS: n = 7 (WT) and n = 6 (Tyk2^-/-, Tyk2^K923E); C. albicans: n = 12 (WT, Tyk2^K923E) and n = 11 (Tyk2^-/-); n: biological replicates. e Representative pictures of the infected skin on day 2 p.i. A Gomori Methenamine-Silver (GMS) staining of the skin sections is shown. Scale bar: 200 μm (top), 50 μm (bottom). Data are representative for sections from 7 mice per genotype. f WT, Tyk2^-/- and Tyk2^K923E mice were infected as described above and wounds were visually monitored for 25 days. g The percentage of mice with wounds is shown. Pooled data from 2 independent experiments are shown. Error bars indicate the standard error (SE), centers for the error bars indicate mean values; n = 13 (WT), n = 11 (Tyk2^-/-) and n = 10 (Tyk2^K923E); n: biological replicates. Statistical analysis was conducted using One-way ANOVA followed by Tukey’s multiple comparison test (b–d) and the Log-rank (Mantel-Cox) test (g). Statistical significance is only given for the comparison between the genotypes (b–d, g). Source data are provided as a Source Data file.