Fig. 7: Protease resistance and mouse plasma stability of synthetic l- and d-monobodies.
From: Development of mirror-image monobodies targeting the oncogenic BCR::ABL1 kinase
a, b Protease resistance of recombinant l-DAM27-XTEN as well as synthetic l- and d-DAM27-XTEN after incubation with (a) pepsin and (b) proteinase K was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). c Plasma stability of synthetic l- and d-DAM27-XTEN. Monobodies labeled with biotin at the N-terminus after incubation with mouse plasma were analyzed by Western blotting and detection of biotin using Streptavidin-IRDye680. A representative blot of three repeats is shown. Source data of (a–c) are provided as a Source Data file.
