Fig. 1: Dilution method showing the effect of treatment on the reactivity of antigen. | Nature Communications

Fig. 1: Dilution method showing the effect of treatment on the reactivity of antigen.

From: Early assessment of antibodies decline in Chagas patients following treatment using a serological multiplex immunoassay

Fig. 1

a Well images of serum samples diluted at 1/25, 1/50, 1/100, 1/200, 1/400, 1/800, 1/1600, 1/3200, 1/6400. These samples were collected at Baseline, 6 months and 12 months after the start of treatment with 300 mg of Benznidazole for 8 Weeks (patient ID3125). The curves showing the effect of treatment of the reactivity of Antigen 3 for (b) patient ID1053 and (c) patient ID3125. D0, Day 0; 6 M, 6 months following treatment; 12 M, 12 months following treatment; DF50, Dilution Factor 50 at which 50% of the original reactivity remains. Bold numbers indicate the 3 selected dilution factors: 50, 400 and 3200. Each plate is imaged and analyzed using a colorimetric reader. An integrated software calculates the pixel intensity for each spot. To establish the net intensity for each antigen, the mean value of the duplicated spots is considered. Net signals correspond to the signal measured from each spot from which the background is subtracted.

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