Table 1 Steady-state kinetic parameters for WT human ASNS and two variants

From: 3D variability analysis reveals a hidden conformational change controlling ammonia transport in human asparagine synthetase

Nitrogen source

L-Glutamine

Ammonia

Enzyme

appKM (Asp)

kcat

appKM (Gln)

kcat

appKM (NH3)

kcat

mM

s−1

mM

s−1

mM

s−1

WT

0.25 ± 0.06

0.83 ± 0.04

0.9 ± 0.1

0.49 ± 0.02

0.5 ± 0.2

0.59 ± 0.05

R142I

0.09 ± 0.03

0.18 ± 0.01

0.01 ± 0.01

0.06 ± 0.01

0.3 ± 0.2

0.54 ± 0.07

R142A

0.20 ± 0.09

0.35 ± 0.03

0.02 ± 0.02

0.10 ± 0.01

1.2 ± 0.4

0.70 ± 0.07

Back to article page