Fig. 3: hPSC-derived ECs, SMCs, and PCs display unique responses to SARS-CoV-2 exposure. | Nature Communications

Fig. 3: hPSC-derived ECs, SMCs, and PCs display unique responses to SARS-CoV-2 exposure.

From: SARS-CoV-2 infection of human pluripotent stem cell-derived vascular cells reveals smooth muscle cells as key mediators of vascular pathology during infection

Fig. 3

Bulk RNA sequencing was performed on RNA isolated from hPSC-derived vascular ECs, PCs, and SMCs 48 hours after virus exposure (MOI = 1). For all volcano plots, effect sizes were estimated using DESeq2, with (two-tailed) p-values computed using the Wald statistic. A full list of differentially expressed genes can be found in the Source Data file. A Volcano plots showing differential gene expression in ECs exposed to live SARS-CoV-2 compared to control uninfected ECs. Gene set enrichment analysis (GSEA)76 was performed on differentially expressed genes to analyze the transcriptional response to infection. Dot plots show gene-sets from the Hallmark collection34 of the MSigDB that were enriched (FDR < 0.05). B Volcano plots showing differential gene expression in SMCs exposed to live SARS-CoV-2 compared to control uninfected SMCs. GSEA was performed on differentially expressed genes to analyze the transcriptional response to infection. Dot plots show gene-sets from the Hallmark collection34 of the MSigDB that were enriched (FDR < 0.05). C Volcano plots showing differential gene expression in PCs exposed to live SARS-CoV-2 compared to control uninfected PCs. GSEA was performed on differentially expressed genes to analyze the transcriptional response to infection. Dot plots show gene-sets from the Hallmark collection of the MSigDB that were enriched (FDR < 0.05).

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