Fig. 2: Broad-spectrum antiviral effects of HNC-1664 against arenaviruses. | Nature Communications

Fig. 2: Broad-spectrum antiviral effects of HNC-1664 against arenaviruses.

From: An adenosine analog shows high antiviral potency against coronavirus and arenavirus mainly through an unusual base pairing mode

Fig. 2

a Luciferase activity of the LASV minigenome after treatment with HNC-1664 (n = 5). Vero E6 cells were cotransfected with LASV mini, LASV NP and L helper plasmids. Luciferase activity was normalized to the vehicle control group. b Luciferase activity of the LCMV minigenome treated with HNC-1664 (n = 5). HEK293T cells were cotransfected with LCMV mini, LCMV NP and L helper plasmids. The procedures for collecting and processing the data are similar to those in panel (a). c Antiviral activity of HNC-1664 against the LCMV Armstrong strain. Representative IFA images showing the LCMV NP protein (green) and nuclei (blue) in Huh-7 cells (n = 3). d Dose-response curve of HNC-1664 for the inhibition of the LCMV Armstrong strain. The data were analyzed by IFA (n = 3). The inhibitory effect of HNC-1664 was calculated relative to the vehicle control group. The IC50 value is presented as the mean ± SD of three independent experiments. All data are presented as the mean ± SD. Statistical significance was assessed by one-way ANOVA with Dunnett’s post-hoc test compared with the vehicle control group. ***P < 0.001, ****P < 0.0001; ns no significance.

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