Fig. 3: METTL1 is required for GC B-cell responses.

a Experimental scheme, which was created with BioRender.com. b Representative images of immunofluorescence staining of spleen sections. B220 (red), CD3 (blue), and PNA (white). c, d Representative flow cytometry plots and frequencies of GL7⁺CD38⁻ GC B cells in the spleens of WT and cKO mice. e, f Representative flow plots and frequencies of IgG1⁺ GC B cells among the GC B cells of the WT and cKO groups. g, h Representative flow cytometry plots and frequencies of NP⁺ B cells in WT and cKO mice. i, j Representative flow cytometry plots and frequencies of NP⁺IgG1⁺ GC B cells in WT and cKO mice. k, l Representative flow cytometry plots, and frequencies of NP⁺ plasmablasts (PB) in WT and cKO mice. m, n Anti-NP25 and anti-NP2 IgG antibodies in the serum were measured via ELISA. o–q Representative flow cytometry plots and frequencies of total memory B cells (p) and NP⁺ memory B cells (q) in WT and cKO mice. WT = 5 biological independent samples, cKO = 4 biological independent samples. The data are presented as the mean ± SEM. Two-tailed unpaired Student’s t test was used in d, f, h, j, l–n, p, q. Source data are provided as a Source Data file.