Fig. 8: CD19 overexpression rescues ETC activity in Mettl1-deleted B cells. | Nature Communications

Fig. 8: CD19 overexpression rescues ETC activity in Mettl1-deleted B cells.

From: Aberrant METTL1-mediated tRNA m7G modification alters B-cell responses in systemic autoimmunity in humans and mice

Fig. 8

a Expression level of CD19 in WT B cells, Mettl1-deleted B cells, and Mettl1-deleted B cells overexpressing Cd19 were measured by western blot. Representative bands were shown. b, c Representative histograms and MFI data of SYK expression were summarized (n = 3 biological independent samples). d, e Representative histograms and MFI data of p-SYK expression were summarized (n = 3 biological independent samples). f Heatmap displaying qPCR data of ETC in WT B cells, Mettl1-deleted B cells, and Mettl1-deleted B cells overexpressing Cd19 (n = 4 biological independent samples). g Western blots displaying protein levels of ETC in WT B cells, Mettl1-deleted B cells and Mettl1-deleted B cells overexpressing Cd19. Representative bands were shown. h, i Seahorse assay measuring mitochondrial respiration of WT B cells, Mettl1-deleted B cells, and Mettl1-deleted B cells overexpressing Cd19 (n = 4 biological independent samples). j, k Representative flow plots and summarized data displaying GC differentiation of WT B cells, Mettl1-deleted B cells, and Mettl1-deleted B cells overexpressing Cd19 (n = 4 biological independent samples). Data are shown as mean ± SEM. One-way ANOVA was used in c, e, i, k. Source data are provided as a Source Data file.

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