Fig. 2: Medial entorhinal cortical input is required for intact phase precession in CA3.
This figure follows the presentation of Fig. 1 but with data from CA3 cells with lesioned MEC inputs and respective controls. a, b Firing patterns of example CA3 cells in control and MEC-lesion rats. Solid green lines in the phase-versus-normalized distance plot indicate significant phase precession (p < 0.05; circular-linear regressions) and dashed green lines indicate a lack of significant phase precession (p > 0.05; circular-linear regressions). Scale bars for LFP: 500 μV and 250 ms, for path: 50 cm. c Phase precession slopes (violin plots; n = 101 control (CTRL) and 158 MEC lesion (LESION) CA3 cells, z-statistic = − 2.34, p = 0.019, MW test) and proportions of phase precessing cells (bar plots; controls vs. lesion, only negative slopes, χ2 = 5.52, p = 0.019; negative and significant, χ2 = 6.26, p = 0.0124, chi-square tests) from the slope-by-cell analysis. The magnitude of the slopes and the proportion of negative slopes were reduced by the MEC lesions. d Slopes for all trains from CTRL(MEC) (left) and LESION(MEC) (right) CA3 cells. Each row depicts the slope values from each of the trains of one cell (yellow and orange ticks), and cells are sorted from top to bottom by their trains’ median slope (black tick when negative, purple tick when positive). e Phase precession slopes (violin plots; n = 101 control and 158 MEC lesion CA3 cells, z-statistic = − 3.91, p = 9.3 × 10−5, MW test) and proportions of phase precessing cells (bar plots; χ2 = 10.50, p = 1.2 × 10−3; chi-square test) from the slope-by-train analysis. Violin plots in panels (c, e): Outline, distribution; shading, negative slopes (inner shading in c, negative and significant slopes); error bars, 1.5 times the interquartile interval above the third and below the first quartile. * p < 0.05, ** p < 0.01, *** p < 0.001. Source data are provided as a Source Data file.
