Fig. 6: DG but not MEC inputs are required for the temporal organization of CA3 cell pairs at the theta-cycle time scale. | Nature Communications

Fig. 6: DG but not MEC inputs are required for the temporal organization of CA3 cell pairs at the theta-cycle time scale.

From: Distinct roles of dentate gyrus and medial entorhinal cortex inputs for phase precession and temporal correlations in the hippocampal CA3 area

Fig. 6

a Pairs of simultaneously recorded CA3 cells with overlapping place fields were selected for the analysis of spiking in shared theta cycles. Cells 1 and 2 are from a control rat, and cells 3 and 4 are from a DG-lesion rat. Each place field is delineated by a contour that corresponds to 20% of the maximum firing rate (dot inside contour, location of peak firing). Each pair of overlapping fields is depicted with the one that is entered first in black and the one entered second in red. Black arrow, running direction. Scale bar, 50 cm. b Phase-position plots of the cell pairs’ spikes (red and black dots, from the cells depicted in red and black in a) while running in the direction indicated by the horizontal arrows (corresponding to the direction in a). c Cross-correlation of the spikes (arrow, peak of the cross-correlation function nearest zero lag; inset, cross-correlogram for a window width of 4 seconds). d Left, Phase shift at the theta-cycle time scale plotted against the distance between place field peaks (solid and dashed lines, linear regression for data from control and DG-lesion rats; CTRL(DG): n = 30 pairs, r = 0.651, p = 9.87 × 10−5, Pearson’s correlation). Right, Proportion of cell pairs where neither, one, or both neurons in the pair displayed phase precession. Although a substantial number of pairs in the DG-lesion group were phase precessing, the phase precession did not yield a relation between pairwise spike-time difference and distance between fields (LESION(DG): n = 14 pairs, r = − 0.187, p = 0.523, Pearson’s correlation). e–h, Same as a–d except that the CA3 data are from the MEC-lesion group and their respective controls. Despite marked deficits in phase precession, there is a strong correlation between spike-time difference and place field distance (CTRL(MEC) n = 19 pairs, r = 0.654, p = 0.0024; LESION(MEC) n = 27 pairs, r = 0.624, p = 5.02 × 10−4; Pearson’s correlations). n.s., not significant, ** p < 0.01, *** p < 0.001. Source data are provided as a Source Data file.

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