Fig. 4: TR-XSS analysis of IsdB:metHb interaction. | Nature Communications

Fig. 4: TR-XSS analysis of IsdB:metHb interaction.

From: Time-resolved X-ray solution scattering unveils the events leading to hemoglobin heme capture by staphylococcal IsdB

Fig. 4

a Schematics of the TR-XSS setup at the ESRF ID09 beamline. X-ray solution scattering patterns are collected as a function of time after rapid mixing of an IsdB solution at a concentration of 270 μM and a Hb solution at equimolar heme concentration by means of a stopped-flow apparatus (BioLogic, SFM-4000). After the two solutions were mixed and transferred to the observation capillary, induced structural changes were probed in the sample using short X-ray pulses (pulse duration = 20 μs) isolated from the X-ray source by means of shutters and choppers. The X-ray scattering pattern generated by each pulse was recorded separately through a fast CCD detector (Rayonix MX170-HS) operated in 8×8 binning in order to achieve readout times as low as 10 ms. b Comparison between the WAXS pattern measured at the earliest available time point (10 ms) and at the latest one (~1 min). c TR-WAXS difference patterns measured at several time-delays from mixing (colormap is used to represent the time course of the reaction, where shorter delays are blue and longer ones are green).

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