Table 1 Kinetic constants calculated by global data fitting to the kinetic model

k_on,1 (M⁻¹ s⁻¹)

(1.4 ± 0.8)⋅10⁶

k_off,1 (s⁻¹)

1⋅10⁻² (*)

STEP 2 (Hb dimerization / activation)

k_on,2 (s⁻¹)

20 ± 2

STEP 3 (IsdB binding to Hb_dim)

k_on,3 (M⁻¹ s⁻¹)

(1.9 ± 0.8)⋅10⁶

k_off,3 (s⁻¹)

0.5⋅10⁻² (*)