Fig. 2: NTD nat_mutant 3D-folds are identical to WT.
a 1H/15N CSD plots for NTD nat_mutants D63G, P67S, and P80R compared to the WT NTD, plotted over the amino acid sequence. Significant CSDs (average + 1SD, threshold indicated by dotted line) are mapped on the surface of the NTD NMR structure (PDB 6YI36) in the respective color. The site of mutation is shown by a red sphere (Cα) and by a red star in the CSD plot. b EMSAs of NTD mutants with a described target RNA, SL4ext (Supplementary Fig. 2c). Protein concentrations are given above. Shown is a representative of two biological replicates (N = 2, see source data for quantification). c Zoom-ins of P67S and WT crystal structures showing the site of mutation. The zoom-in was set to comprise regions of significant CSDs according to panel (a) (see also Supplementary Fig. 2a). d Comparison of MST-derived KD values of WT and three nat_mutants D63G, P67S and P80R for viral Ext RNA (3’-Cy5 labeled, see also source data). The transition point (50% bound) for RNA-binding deficient mutant R107A is indicated by dotted lines (see source data for the full curve). Data are presented as mean values +/− SD from three biological replicates (N = 3), each measured in duplicate. e, f Zoom-ins of the P80R and WT (e), and D63G and WT (f) crystal structures showing the site of mutation. The zoom-in area was set to comprise regions of significant CSDs according to panel (a) (see also Supplementary Fig. 2a). Contacts that are affected by the mutation are indicated (green – salt bridges, orange – H-bonds).
