Table 1 Overview of crystal structures in this study with their PDB codes and respective specifications along with NMR assignment IDs. See Supplementary Table 3 for statistics and Supplementary Table 1 for the backbone assignment details

From: A core network in the SARS-CoV-2 nucleocapsid NTD mediates structural integrity and selective RNA-binding

NTD_varianta

PDB code

Resolution in Å

RMSDc in Å

Number of chains

Average chain RMSDd in Å

Chain used for depictione

BMRB IDf

Sequence coverage (% of all possible backbone amides)

NTD_WT

9EXB

2.30

–

4

0.581

C

345116

–

NTD_D63G

9F83

1.70

0.668

4

0.476

D

52471

98.5

NTD_P67S

9EZB

1.60

1.047

4

0.667

A

52472

98.5

NTD_P80R

9F7A

1.90

0.898

1

–

A

52473

98.5

NTD_A119S

9F5L

2.36

0.592

4

0.399

B

n/ag

n/ag

NTD_E136D

9EVY

1.55

0.766

4

0.627

A

n/ag

n/ag

NTD_P151S

9FBG

2.54

1.776

16

0.775

H

n/ag

n/ag

NTD_Q58Ib

9F5J

2.20

4.552

2

0.331

A

52469

98.5

NTD_S105I

9F7C

2.00

3.198

1

–

A

52474

94.2

NTD_Y109A

9EWH

1.93

0.674

4

0.727

C

52470

97.8

  1. aNTD boundaries for X-ray crystallography span residues 41–174.
  2. bNTD_Q58I was crystallized using a construct with boundaries from 44 to 180.
  3. cRMSD for mutants with the WT crystal structure from this study; PDB 9EXB.
  4. dAverage RMSD for all chains within one asymmetric unit.
  5. eSee Method section for details on the selection of chain usage in figure panels.
  6. fBackbone chemical shift assignments have been deposited at the BMRB for domain boundaries 44-180.
  7. gNTD nat_mutants with only local CSD compared to WT. For details, see the Methods section.
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