Table 2 List of DNA oligonucleotides used in this study

Name	Sequence (5’ - > 3’)	Usage
xFW	GGTCTCGAGAATCTTTATTTTCAGGGCAGCCGTCCACAAGGTCTCCCTAAC	Cloning of NTD_xtal_WT(Gibson assembly)
xRV	GTTAGCAGCCGGATCCCGACCCTTATTCTGCATAAAAGCCCTTTGGGAGCGTTGTGCCTTG	Cloning of NTD_xtal_WT(Gibson assembly)
D63G_FW	CAGCATGGCAAAGAAGGGTTGAAGTTTCCCCGGG	SDM Mutagenesisa
D63G_RV	GGTTAGGGCCGTGAACCAGG	SDM Mutagenesis^a
P67S_FW	CAAAGAAGATTTGAAGTTTAGTCGGGGACAGGGGGTTC	SDM Mutagenesis^a
P67S_RV	CCATGCTGGGTTAGGGCC	SDM Mutagenesis^a
P80R_FW	GAACAGCTCGCGGGATGATCAAATTGG	SDM Mutagenesis^a
P80R_RV	GTGTTGATCGGAACCCCCTG	SDM Mutagenesis^a
A119S_FW	GTCCCGAATCAGGCCTGCCGTATG	SDM Mutagenesis^a
A119S_RV	CAGTGCCCAGATAGTAAAAGTACCATC	SDM Mutagenesis^a
E136D_FW	GTCGCAACCGACGGTGCGCTCAATAC	SDM Mutagenesis^a
E136D_RV	CCAAATAATGCCGTCTTTATTAGCACC	SDM Mutagenesis^a
P151S_FW	CACTCGCAACTCGGCGAATAACG	SDM Mutagenesis^a
P151S_RV	CCAATGTGGTCCTTCGGGG	SDM Mutagenesis^a
Q58I_FW	GTTCACGGCCCTAACCATTCATGGCAAAGAAGATTTGAAG	SDM Mutagenesis^a
Q58I_RV	CAGGAAGCCGTGTTATTAGGAAGTCC	SDM Mutagenesis^a
S105I_FW	GAAAGACTTAATTCCGCGATGGTAC	SDM Mutagenesis^a
S105I_RV	ATTTTCCCATCGCCACCA	SDM Mutagenesis^a
Y109A_FW	GAAAATGAAAGACTTAAGTCCGCGATGGGCGTTTTACTATCTGGGCACTG	SDM Mutagenesis^a
Y109A_RV	CCATCGCCACCACGGATACG	SDM Mutagenesis^a
R107A_FW	GACTTAAGTCCGGCGTGGTACTTTTACTATCTG	SDM Mutagenesis^a
R107A_RV	CAGATAGTAAAAGTACCACGCCGGACTTAAGTC	SDM Mutagenesis^a
W108G_FW	GAAAGACTTAAGTCCGCGAGGGTACTTTTACTATCTGGG	SDM Mutagenesis^a
W108G_RV	ATTTTCCCATCGCCACCACGGATAC	SDM Mutagenesis^a
F171G_FW	CCTGCCTAAAGGTGGTTATGCCGAAGGCTCCCG	SDM Mutagenesis^a
F171G_RV	GTGGTACCCTGTGGCAGTTGCAG	SDM Mutagenesis^a

^aSDM = site-directed mutagenesis using primers designed with NEBaseChanger® following the protocol described in the methods section.

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