Fig. 3: The four mtAP2s are associated with the mitoribosomes.
From: Apicomplexan mitoribosome from highly fragmented rRNAs to a functional machine
a Schematics for the mtAP2-(1 to 3)-HA immunoprecipitations ± MgCl2. b Volcano plots showing the enriched proteins via mtAP2-(1 to 3)-HA immunoprecipitations ± MgCl2, detected by mass spectrometry. Homologs of universal conserved mtRPs (LSU and SSU) and lineage-specific mtRPs (LSUl and SSUl) are represented by colored dots. The cutoff for enriched proteins was a p-value < 0.05 and Log2FC ≥ 3. X-axis shows log2 fold change, Y-axis shows -log10(P-value). c Schematic representation and summary table of mtRPs based on the differential protein enrichment from various mtAP2s-IPs ± MgCl2. d A schematic representation of the mitoribosome profiling assay. e Distribution of mitoribosomal rRNAs after sucrose gradient sedimentation of T. gondii cellular extracts expressing uL4m-FLAG and mtAP2-3-Ty, supplemented with RNase inhibitors ( + RNasIn) were analyzed. Fourteen fractions were collected, and mt-rRNA content was quantified. SSUA represented the small subunit, and LSUA represented the large subunit. Results were normalized to TgACT levels. Western blot analysis (Top) is included. Two independent experiments. f–i The distribution of uL4m, uS5m, bL27m in the mtAP2-(1 to 4) iKD parasites in the presence or absence of ATc treatment on a 10–50% sucrose gradient fractions. No corresponding western blot for mtAP2-4 is available due to unsuccessful attempts to tag this protein. Immunoblot for catalase instead. Two independent experiments. Source data are provided as a Source Data file.
