Fig. 3: Elevated NETosis occurs in aortic lesions and peripheral blood from patients with AD. | Nature Communications

Fig. 3: Elevated NETosis occurs in aortic lesions and peripheral blood from patients with AD.

From: Integrated multi-omics profiling reveals neutrophil extracellular traps potentiate Aortic dissection progression

Fig. 3

A Single-cell sequencing data of NETs-associated gene module score of dissected aortas (left) and NETs-associated gene module score of each cell cluster (right). B Representative immunofluorescence images of NETs identified by co-localization of MPO (red) and CitH3 (green). Scale bars = 1 mm. C Zoom-in immunofluorescence images of NETs determined by co-staining of MPO (red) and CitH3 (green). Scale bars = 100 μm. M indicated media, A indicated adventitia, and the white dotted line indicated the boundary between media and adventitia. D NETs associated markers as detected by ELISA in the plasma from patients with AD (n = 187) and healthy individuals (n = 59) (p < 0.001; p = 0.0069; p < 0.001; p < 0.001; p = 0.2177; p = 0.0140, respectively). Source data are provided as a Source Data file. Values are expressed as means ± SD. A two-sided statistical significance was set at *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by Mann–Whitney U test. NETs: neutrophil extracellular traps; CitH3: citrullinated histone H3; cf-DNA: cell-free DNA; MPO: myeloperoxidase; NE: neutrophil elastase; IL-1β: interleukin-1β; IL-6: interleukin-6.

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