Fig. 3: The functions and hematopoietic support capacity of BM EPCs from CR patients were further enhanced by NAC in vitro. | Nature Communications

Fig. 3: The functions and hematopoietic support capacity of BM EPCs from CR patients were further enhanced by NAC in vitro.

From: Bone Marrow Endothelial Progenitor Cells remodelling facilitates normal hematopoiesis during Acute Myeloid Leukemia Complete Remission

Fig. 3

a ROS levels in cultivated AML EPCs and CR EPCs demonstrated a decrease after NAC treatment. b The apoptosis ratio was lower in CR EPCs treated with NAC, indicating improved cell survival. c CD144 expression, a key marker for EPCs, was significantly elevated in CR EPCs following NAC exposure. d ZO-1 expression, an indicator of cellular integrity, showed an improvement in CR EPCs with NAC. a–d Ten paired samples from each of the groups (N = 10 per group, n = 1 per sample) were collected on different days, with one sample per group analyzed together on the same day. e BM EPCs were characterized by double staining with DiI-Ac-LDL(red) and FITC-UEA-1(green), as seen in the merged yellow signal. f On Day 7 of culture, the number of double-positive EPCs per field of view was quantified using DiI-Ac-LDL and FITC-UEA-1 staining. g Representative images depicted the migration of BM EPCs, with NAC-treated CR EPCs showing increased migration. h Quantification of migrated EPCs confirmed this observation. i BM EPCs formed tube-like structures, and j the tube formation assay revealed a higher number of tubes per field of view in the NAC-treated group. e–j Six paired samples from each of the groups (N = 6 per group, n = 3 per sample) were collected on different days, with one sample per group analyzed together on the same day. k ROS levels in HSCs cocultured with CR EPCs were reduced after NAC intervention. l The apoptosis rate of HSCs was lower in the presence of NAC-treated CR EPCs. k, l Ten paired samples from each of the groups (N = 10 per group, n = 1 per sample) were collected on different days, with one sample per group analyzed together on the same day. m The colony-forming unit(CFU) plating efficiency of HSCs(from HDs) was significantly enhanced after coculture with NAC-boosted CR EPCs (N = 10 per group, n = 3 per sample). All scale bars represent 200 μm, with an original magnification of 10×. N represents biological replicates; n represents technical replicates. Statistical analyses were conducted using paired t-tests. The data are presented as the means ± SEMs. The statistical tests were two-sided and no adjustments were made for multiple comparisons. Source data are provided as a Source Data file.

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